rabbit anti solute carrier family 7 member 11  (Proteintech)

Journal: Bioactive Materials

Article Title: Immunomodulatory copper-based polyphenol nanozyme for diabetic infectious wound healing via NIR amplified cuproptosis bacteriostat in synergy with ferroptosis inhibition anti-inflammation

doi: 10.1016/j.bioactmat.2025.08.042

Figure Lengend Snippet: In vitro anti-inflammation abilities and the relative mechanism. A) Cell viability of RAW264.7 incubated with CuCl 2 , EA and CE of different concentrations ranging from 2 to 500 μg/mL. B) Inflammatory factors (IL-6, TNF-α and IL-1β) expression levels of the supernatant of treated RAW264.7 by ELISA. C) Relative genes expression levels of treated RAW264.7 by RT-qPCR: IL-6, IL-1β, iNOS and IL-10. D) IL-1β expression levels of treated RAW264.7 by fluorescent microscope. E) Ferroptosis pathway related DEGs by KEGG enrichment analysis. F) Ferroptosis pathway related differential biological functions by GO enrichment analysis. G) The relative pathway related proteins (GPX4 and SLC7A11) expression levels of treated RAW264.7 by WB, and the corresponding quantified results: relative GPX4/actin and SLC7A11/actin ratios (H). I) GPX4 expression levels of treated RAW264.7 by fluorescent microscope. J) Nrf-2 expression levels of treated RAW264.7 by fluorescent microscope. Scale bar = 200 μm. (“∗” symbol compared with normal group, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 and ∗∗∗∗p < 0.0001).

Article Snippet: After blocking with blocking buffer (MEIMIAN, China) for 2 h, and rinsing with tris buffered saline with Tween-20 (TBST, Servicebio, China) for 3 times, the membranes were incubated with the primary antibody ( anti -GPX4, anti-solute carrier family 7 member 11 (SLC7A11) or anti-actin, Proteintech, China) at 4 °C respectively.

Techniques: In Vitro, Incubation, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Microscopy